| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018918 |
| Chromosome: | chromosome 16 |
| Location: | 5757602 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680500 | EFH10 | (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair; EF-hand Calcium binding protein, may be centrin-related | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAGCGCCGCGCCAGGCGACACGCCGGAGCCCCAGCCGAAGGGGGCCGC |
| Internal bar code: | CGAGGTACAGAGTCTAAGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3730 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAAGCAGGTGGGAGGAG |
| Suggested primer 2: | TCATTGACACCGCTCTTGCT |