Insertion junction: CLIP2.018929_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.018929
Chromosome:chromosome 4
Location:2417587
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g221500 SMM13 S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGTGCGACGCTTCGGTCGGCGCTGTCGATTTGCCCATGCGCAGCGCCA
Internal bar code:GTGACGACGCCTTTCACCTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1067
LEAP-Seq percent confirming:93.3333
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCACATCGCCCGTCTAA
Suggested primer 2:TGCGATTAAAGCATGCGTCG