| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018947 |
| Chromosome: | chromosome 2 |
| Location: | 4896459 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109800 | CPLD32,FAO3 | conserved FAD dependent oxidoreductase; (1 of 1) PTHR13847//PTHR13847:SF184 - FAD NAD BINDING OXIDOREDUCTASES // FAD-DEPENDENT OXIDOREDUCTASE-LIKE PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAACGCACACGCACCAACAGCTGCCACGCCGCCGCCACGCCCGCCAG |
| Internal bar code: | GTATAGACTCGGGTCGAGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1590 |
| LEAP-Seq percent confirming: | 86.8421 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTAGAGGTTTGCAGCGA |
| Suggested primer 2: | AGACCATGGCGCCATACAAA |