Insertion junction: CLIP2.018962_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.018962
Chromosome:chromosome 9
Location:2683262
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g391901 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCGCACCCGCAGAGCCCAGCGGCAGGAGCTCACGCGCCTGTTGCGCC
Internal bar code:TACGAAAGCTTCCGAGGTATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2356
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGAAGTCATCAGCGGCTC
Suggested primer 2:GTACTGCTCCGTCATCTCCG