Insertion junction: CLIP2.018992_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.018992
Chromosome:chromosome 6
Location:3072325
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g275050 GGH2 (1 of 2) 3.4.19.9 - Gamma-glutamyl hydrolase / Pteroyl-poly-alpha-glutamate hydrolase; Gamma-glutamyl hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCACACTGTTGCTCGGGCGGGTTCGCGGTCTCGACTCGATAGGTGCA
Internal bar code:ATGCGGGCCAAATCCGTACGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1114
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CGATAATGGGGCGCATGTTG
Suggested primer 2:TGTTCCAGCCATTTTGTGCG