| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019002 |
| Chromosome: | chromosome 2 |
| Location: | 2943290 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095250 | (1 of 1) 6.6.1.2 - Cobaltochelatase / Hydrogenobyrinic acid a,c-diamide cobaltochelatase | intron | |
| Cre02.g800209 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGACTGGATGGCTCTGCACGTGTCAGCCCGCTGCATGTGCTGACTGTT |
| Internal bar code: | GTCGTTGGAAGCTACGAAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3928 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCTGGTGCTCCATCATT |
| Suggested primer 2: | GTACTGCAGGACTTTCGCCT |