| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.019003 |
| Chromosome: | chromosome 14 |
| Location: | 3723765 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631500 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCCTTGACCCCCCGAGCGGCATACGCCTCGACCATGCCGTTGAGGTT |
| Internal bar code: | TCGAGGTTCAAAGTCTCGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 19.2308 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCGCCAGCCATAGTAAG |
| Suggested primer 2: | ACCACTGGATCAGGGCAATG |