Insertion junction: CLIP2.019034_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.019034
Chromosome:chromosome 12
Location:2994523
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g501950 PPP39 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAACATGCAACCCCGCCAGCTCACCTGCTGCGTGGTCATGACGTTCCA
Internal bar code:GTCTGGTCCGTTACAGTCTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:953
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AACAACAACCTTTGCGCCTC
Suggested primer 2:TCTCCTCATCTCCGTCTGCA