| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.019043 |
| Chromosome: | chromosome 16 |
| Location: | 4333857 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668850 | A2,MTA2 | Hydroxyproline-rich glycoprotein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATTGGCATTCTACGGGGCGTTGTAGCGCAGCGCTTGCATGGTGCTGA |
| Internal bar code: | GGTACCGATCGGCACTTTAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4345 |
| LEAP-Seq percent confirming: | 98.3333 |
| LEAP-Seq n confirming: | 118 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 120 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTAATCAAGCTTCCGCGC |
| Suggested primer 2: | CGTAATGATGGGAGTCGGGG |