| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.019074 |
| Chromosome: | chromosome 10 |
| Location: | 749234 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422700 | SMM35 | (1 of 25) IPR013216//IPR029063 - Methyltransferase type 11 // S-adenosyl-L-methionine-dependent methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACAGACGATCGTGAAATCCTTCCTTGTAAATCTCATAATCGCTGGCGC |
| Internal bar code: | GGTGGATAGTTCCGACTAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCTGCCTGAAGGATACC |
| Suggested primer 2: | TCCTCTACCGCTGTGTACGA |