| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.019098 |
| Chromosome: | chromosome 17 |
| Location: | 3999397 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728300 | FAP226,CAL6 | Flagellar Associated Protein 226; (1 of 3) 3.4.22.54 - Calpain-3 / Muscle-specific calcium-activated neutral protease 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTTGCCGGGTCCTTCTCGTCCAGCATGTACAGCGCCGCGCGGTTGAG |
| Internal bar code: | CGGGCAAACGGAAATGGGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 933 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTCTCCCCTCTGCTCCT |
| Suggested primer 2: | CTCCTTGTGCGAAGGTTCCT |