Insertion junction: CLIP2.019099_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.019099
Chromosome:chromosome 1
Location:207451
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g001300 ankryin repeat containing protein; (1 of 1) IPR000782//IPR006626 - FAS1 domain // Parallel beta-helix repeat CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCTACTGCTACTGCTGCTGGCGGCGACGGTGGCGGCGGCAGCGTGTG
Internal bar code:TCTCGTAGTCGTCGATTGAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:421
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTTCCCCACCATCATGAC
Suggested primer 2:GATACCCCGACATCTACCCG