Insertion junction: CLIP2.019119_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.019119
Chromosome:chromosome 12
Location:9543582
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g540050 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCGCCGCTGCTGGTTGCGTCGGCGGTGGTTGCGCCGTCCGCATCCGC
Internal bar code:AGTTATCTAGGTAACTGACGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1439
LEAP-Seq percent confirming:77.4194
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAACAGCACACGCATGAA
Suggested primer 2:GGCGTATGACGAGGAGGAAG