| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.019125 |
| Chromosome: | chromosome 6 |
| Location: | 4797047 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279900 | MOT14,VFL3 | Variable Flagella Number 3; (1 of 1) K16755 - coiled-coil domain-containing protein 61 (CCDC61) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCCCCGCCCCCGGCTGTTGCTGCGGCTGTCCGCTCCCCGTGTTGGCG |
| Internal bar code: | TAATCTAGATGCTATAGAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 461 |
| LEAP-Seq percent confirming: | 21.0526 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAGAGGTGTTGCTAGTG |
| Suggested primer 2: | CCTTCTATGCGTGCTAGCGA |