| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.019127 |
| Chromosome: | chromosome 3 |
| Location: | 5901939 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188750 | HEL21 | (1 of 1) K13177 - ATP-dependent RNA helicase DDX1 [EC:3.6.4.13] (DDX1); DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGTTGATGCAGATGCGGCCAGCCATGTCCTTCACCTCGGGCGAGTG |
| Internal bar code: | TTTACGGCCGGATAGCGACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3525 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCACATGTGGTCCAACAC |
| Suggested primer 2: | GGGGTGTGAGATGAAGGGTG |