| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.019141 |
| Chromosome: | chromosome 12 |
| Location: | 5517192 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530350 | CrZIP12,ZIP10,IRT2 | Iron-nutrition responsive ZIP family Fe transporter; (1 of 5) K07238 - zinc transporter, ZIP family (TC.ZIP, zupT, ZRT3, ZIP2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGGGTCGGTATGGCACACAGGAAGGATGCGCAGGAGGGCCAGGGGCA |
| Internal bar code: | ATACGGGTAGAAATTGGAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4744 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGAGGACTTGCTGCTTA |
| Suggested primer 2: | ATACAACCGGCGTTCCTGTT |