Insertion junction: CLIP2.019148_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.019148
Chromosome:chromosome 7
Location:4146387
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g342450 SEE3 Putative splicing endonuclease positive effector; (1 of 3) K10706 - senataxin [EC:3.6.4.-] (SETX, ALS4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTACGCTCCCAACCCCGCAGTGACTGCTCCCCGCTCCGCCCACGGCAC
Internal bar code:GTGCGAAGGGTTGATGCTGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:317
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAACAGTTGGGAGTGCTT
Suggested primer 2:CCGCGAACAAACACACACAT