| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.019156 |
| Chromosome: | chromosome 3 |
| Location: | 3627601 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168250 | (1 of 6) PF06701 - Mib_herc2 (MIB_HERC2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCAAGGCTAGCGAGGCTTACTTCGTGTGGCTGGCGGGTGCGTGCGTGC |
| Internal bar code: | CTGTAGTGTGCGGCTATAGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1430 |
| LEAP-Seq percent confirming: | 88.5714 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTCCCTTTGTGTTGTGG |
| Suggested primer 2: | GTGCGTGTGTACCTGTCTCA |