Insertion junction: CLIP2.019168_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.019168
Chromosome:chromosome 8
Location:1409823
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g364351 FAL12 Protein of unknown function; (1 of 3) 2.4.1.68 - Glycoprotein 6-alpha-L-fucosyltransferase / Guanosine diphosphofucose-glycoprotein fucosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATAGAGCTCCGCCGCTTTTACGTCGCGGCCATAACAAGGACATGTTAA
Internal bar code:CAATTTATACAAACGTTCGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:476
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGTTACAGGCACGTCTGT
Suggested primer 2:CGCATTCGAACATGTAGCGG