| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.019181 |
| Chromosome: | chromosome 3 |
| Location: | 6810793 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197250 | (1 of 1) PTHR13948//PTHR13948:SF25 - RNA-BINDING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCCGGGGACGGGATGGCCGTGGTGGTGGCGGCCGCGAAGGAGGTGTG |
| Internal bar code: | CTGGTTGGGTTCAGTTCATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1625 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCTCCGCAAGTGTGTTA |
| Suggested primer 2: | GTCGCATAGCATGTGGGGTA |