Insertion junction: CLIP2.019215_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.019215
Chromosome:chromosome 8
Location:3728530
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g380550 SDR14 (1 of 2) IPR002347//IPR013968 - Glucose/ribitol dehydrogenase // Polyketide synthase, ketoreductase domain; Short-chain dehydrogenase/reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGAGGGTGACGCCTTATCCATTAGGCCACTCGGCCTCGAGTGGGTGCC
Internal bar code:TATATTGGAAGTATTGACTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:620
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTATCAAGGACGGCAGGG
Suggested primer 2:AAGCGCGATAACCAAATGGC