Insertion junction: CLIP2.019216_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.019216
Chromosome:chromosome 1
Location:2812963
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g017350 RMT3 Putative protein N-methyltransferase; (1 of 5) IPR000104//IPR001214 - Antifreeze protein, type I // SET domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATCCCCAGGGAAGGGGCCGTCGCTGTGCATGTGAAGCGCGCAGGCGCG
Internal bar code:CATTTGCCCTCACACTCAGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1644
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGGACACACGCTGTACA
Suggested primer 2:GTCGCCCGGAAGTGAAAAAC