| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.019223 |
| Chromosome: | chromosome 11 |
| Location: | 3071547 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g474950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGTTCACTTGTGGGCTCGCCGCCAAGGTTGGTGCATTGGGTTTCGGG |
| Internal bar code: | GTAATTCCCGAGGTTCAGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3129 |
| LEAP-Seq percent confirming: | 95.7447 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGTTAGCATAGCACTGC |
| Suggested primer 2: | TGCTGAAGTTGGCATCGACT |