| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.019236 |
| Chromosome: | chromosome 5 |
| Location: | 863188 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246200 | (1 of 2) PTHR24347//PTHR24347:SF159 - SERINE/THREONINE-PROTEIN KINASE // SERINE/THREONINE-PROTEIN KINASE STK11 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCGATTCCCCAAGCCGCTGCCGACACCACCGCTATTGTGTGGCGTCA |
| Internal bar code: | GCTCGAAGGAGATTTGTCTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1079 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGTTCTCTGCAGCTGAT |
| Suggested primer 2: | GGTTGTCAAGGTGCGTGAAC |