Insertion junction: CLIP2.019238_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.019238
Chromosome:chromosome 17
Location:6621740
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g745547 (1 of 1) PF05182 - Fip1 motif (Fip1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGCGCTCGCGTTCTCGCTCCCGTTCCCGCCGCTCGCGCTCCCGCTCGC
Internal bar code:ATCTAAATCGGCATGTGTCTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2123
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGCGGCCTATTTATGACT
Suggested primer 2:GACTCGCTGTTCTCTGACCC