Insertion junction: CLIP2.019260_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.019260
Chromosome:chromosome 12
Location:7220056
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g559053 CSB50 {"Probable transposon-derived protein of Chlamydomonas-Specific family B; (1 of 20) IPR010095//IPR013083 - Transposase IS605, OrfB, C-terminal // Zinc finger, RING/FYVE/PHD-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATTCAACCCACCCACCTTGGAGGTCTTGTATTCATCGATGATGAACAC
Internal bar code:GGTTCGTTTGGAACCAAGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2226
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:TTATGGAAACCGGGTCAGCC
Suggested primer 2:TAGGCCCCGACATTTTGGAC