Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.019270 |
Chromosome: | chromosome 12 |
Location: | 5842211 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g533250 | (1 of 2) IPR000104//IPR001394//IPR028889 - Antifreeze protein, type I // Peptidase C19, ubiquitin carboxyl-terminal hydrolase // Ubiquitin specific protease domain | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCGACACTGCGCCCGCTGGCAGCAGCCCACCGCCCGTGTCTCCAGC |
Internal bar code: | TATTTTGGGTTAATCTTCCTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1241 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 11 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCCAAACGCTCCCTAGTTT |
Suggested primer 2: | AAGGGGCAGGAGAGGGATAG |