Insertion junction: CLIP2.019270_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.019270
Chromosome:chromosome 12
Location:5842211
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g533250 (1 of 2) IPR000104//IPR001394//IPR028889 - Antifreeze protein, type I // Peptidase C19, ubiquitin carboxyl-terminal hydrolase // Ubiquitin specific protease domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCCGACACTGCGCCCGCTGGCAGCAGCCCACCGCCCGTGTCTCCAGC
Internal bar code:TATTTTGGGTTAATCTTCCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1241
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCAAACGCTCCCTAGTTT
Suggested primer 2:AAGGGGCAGGAGAGGGATAG