| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019272 |
| Chromosome: | chromosome 2 |
| Location: | 1682986 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085750 | (1 of 8) PF13416 - Bacterial extracellular solute-binding protein (SBP_bac_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGATCCAGCGCATGCGACTGATGCACCCACGCACCCACACATGCATGC |
| Internal bar code: | AGATCTTTTGTATCACGTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6947 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGAGGTAAGGAGCTGGG |
| Suggested primer 2: | TGTCTCGCGTGCAGTTTACT |