| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.019274 |
| Chromosome: | chromosome 12 |
| Location: | 4352878 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519180 | EFT1a,PSRP7,EFT1 | Chloroplast elongation factor Ts-like protein; (1 of 1) K02357 - elongation factor Ts (tsf, TSFM) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTGTGACCACGATATACAGGTGCTGGCAAAGGAGCGCGAGGTGGAGA |
| Internal bar code: | TAGCTCCCTATCCTCGCCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2579 |
| LEAP-Seq percent confirming: | 89.6552 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGGCTCCTCCTTCTTGG |
| Suggested primer 2: | CGATGTGAACAGGCCAGACT |