Insertion junction: CLIP2.019286_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.019286
Chromosome:chromosome 8
Location:717017
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g360564 ERM3 (1 of 13) PTHR13018//PTHR13018:SF5 - PROBABLE MEMBRANE PROTEIN DUF221-RELATED // PHOSPHATE METABOLISM PROTEIN 7; ERD4-related membrane protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCATCCCATCTCATCCCATCTCGCTCGCTCGCTGGCTCGCGCCCACCTA
Internal bar code:GGAGTATGAACTAACGCTCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:2350
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCGTTTTCGTTGGTGTT
Suggested primer 2:CACAATGTTGCCTGCTGCTT