Insertion junction: CLIP2.019315_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.019315
Chromosome:chromosome 13
Location:3233529
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g585550 HAP2 (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase; Putative haloperoxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCCGCTGTTCCCGAGTACGGCCAGGTGGAGCAGGACTTCATCCTGTAC
Internal bar code:AGGCCACGAAGTGTTTTGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4411
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:96
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:96

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCAGTTGATTACCGCGG
Suggested primer 2:CACCCAAGACCCCATCTCAC