Insertion junction: CLIP2.019354_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.019354
Chromosome:chromosome 5
Location:2796755
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g241050 (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTTACAAGGACAGGAATGCACGTCGTGCTCACCACCTTGTAACCACA
Internal bar code:AACTCGCGCGCGCGACTCAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2385
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGGCGTTCAGGTTGATA
Suggested primer 2:CGCACTGCATGTCATGAAGG