| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019360 |
| Chromosome: | chromosome 6 |
| Location: | 45071 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g248900 | (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype | intron | |
| Cre06.g248950 | (1 of 3) K11855 - ubiquitin carboxyl-terminal hydrolase 36/42 [EC:3.4.19.12] (USP36_42) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTTATTTGCAAATTCGCGGTCTTCTGCAGTCACGCTCACCATTAGCCA |
| Internal bar code: | TACTGCGGTCGGAGATAGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3555 |
| LEAP-Seq percent confirming: | 72.2222 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGTATGATCGCGTACACA |
| Suggested primer 2: | CCAGCTCTTCAAGGCAGTCA |