Insertion junction: CLIP2.019363_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.019363
Chromosome:chromosome 13
Location:1574200
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g573100 MAM3C (1 of 5) K16302 - metal transporter CNNM (CNNM); Protein putatively involved in mitochondrial biogenesis CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCCACAAGATCGCGTTCAAGTCCATGACGCCGCTGGACAAGGTGTTCA
Internal bar code:CTTTTGCTTATCGTATAAAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:361
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTTGACGGTTGGCAGTTG
Suggested primer 2:CATAGCCACCACACCCTGTT