Insertion junction: CLIP2.019378_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.019378
Chromosome:chromosome 2
Location:7625196
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g147850 CLPY2,HSLU2,HUV2 (1 of 2) K03667 - ATP-dependent HslUV protease ATP-binding subunit HslU (hslU); Peptidase subunit of mitochondrial HslUV protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCATTCAGCCTCGTCTCCGAGGTGCACCCGCATGCGCTGCCATGCTCC
Internal bar code:ATGTTTGTGTTCGAAATGGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:823
LEAP-Seq percent confirming:70.5882
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGCGTGTGTTTGTACAAG
Suggested primer 2:TTGACAAGATCGTGGAGCCC