Insertion junction: CLIP2.019407_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.019407
Chromosome:chromosome 2
Location:7705279
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g147050 (1 of 7) PF05517 - p25-alpha (p25-alpha); P25 Alpha Domain Containing Flagellar Associated Protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCAACGAACATCACGCCATTCCGAAGATTTCCCCGAAATCTTGTGTA
Internal bar code:TCGAGACTATAAATACTGGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2687
LEAP-Seq percent confirming:80.6452
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:AATCTTCTCCTGCAGGGTGC
Suggested primer 2:TTCATCGCCTTTGCCAGCTA