Insertion junction: CLIP2.019413_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.019413
Chromosome:chromosome 8
Location:3876460
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g381800 (1 of 2) 5.3.3.2 - Isopentenyl-diphosphate Delta-isomerase / Methylbutenylpyrophosphate isomerase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGTGCACACGGCTGCCGCCCCCCCTCCCTCCCTCCAGGGCATCTGGCA
Internal bar code:TCTTGTGTTTCGGACAGTTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:487
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGCGTTTGTAAGGGTTT
Suggested primer 2:CGTATGTGAACTGCATGCCG