| Insertion cassette: | CIB2 | 
| Side of cassette: | 5' truncated? | 
| Strand: | + | 
| Strain: | CLIP2.019442 | 
| Chromosome: | chromosome 12 | 
| Location: | 5128737 | 
| Confidence (%): | 80 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre12.g801426 | (1 of 726) IPR011009 - Protein kinase-like domain | intron | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCATGCTGCGTGCCATGGCGCTCTTCATCAAAGCACCTGAACGCTCG | 
| Internal bar code: | ATGAAGTCGGATTGTGGGACAT | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2269 | 
| LEAP-Seq percent confirming: | 2.0 | 
| LEAP-Seq n confirming: | 1 | 
| LEAP-Seq n nonconfirming: | 49 | 
| LEAP-Seq n unique pos: | 50 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCAAGCAGCACGGTCTT | 
| Suggested primer 2: | TCAGGTGTGCCGCAATGTAT |