| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.019460 |
| Chromosome: | chromosome 16 |
| Location: | 7382888 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680117 | (1 of 1) K15692 - E3 ubiquitin-protein ligase RNF13 [EC:6.3.2.19] (RNF13, RZF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATAACTGTACCCGGCATTCTACTGTAACGAGTTAACACGTAGGTAATGG |
| Internal bar code: | CATCTAGATTTGTTGCCGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 21.4286 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTACACGTTTGCAGGC |
| Suggested primer 2: | TGGTTTTGGGACTGCACTGT |