| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019480 |
| Chromosome: | chromosome 12 |
| Location: | 2628476 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505900 | TIM50 | (1 of 1) K17496 - mitochondrial import inner membrane translocase subunit TIM50 (TIM50) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGAATAAGGGCTAAGCTGCTTAACCCCAGCCAGTGTTATGCCGATCCT |
| Internal bar code: | GTTCCCGAGTCGTCGGAGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4174 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTGGGTGGATGCCAAT |
| Suggested primer 2: | TGGCGCCATACCGTATGTAC |