| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.019486 |
| Chromosome: | chromosome 12 |
| Location: | 5112854 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525990 | HCS5 | Putative holocytochrome-c synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGACCCACCATGCCCAACACCCTGGCTCTCCCCCAGTCCATAAACAC |
| Internal bar code: | TAATCAATATTTACTCGTGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4042 |
| LEAP-Seq percent confirming: | 2.15054 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCGACTGAAACCGCTTG |
| Suggested primer 2: | ATGTGCTCCCTTAACGGTCG |