Insertion junction: CLIP2.019521_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.019521
Chromosome:chromosome 14
Location:2329290
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g622901 (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGAAGAAACAAAACATGTCATAGTAATCGGCTGGAATACGACCATGTA
Internal bar code:CTTGTTCAAACAATTAAACTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1598
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ACTACGATACCCCAAGCCCT
Suggested primer 2:GTTCGTGTCTGTCCAGTCGT