| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.019527 |
| Chromosome: | chromosome 16 |
| Location: | 6581650 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674050 | ADH12 | Alcohol dehydrogenase; (1 of 1) 1.6.5.5 - NADPH:quinone reductase / Zeta-crystallin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCTCCGTCACTCGCTCCAGAGGGAACCTGCGGAGCAGCGGGGTTGGG |
| Internal bar code: | TTCGCTAGGAAATCTACCGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2343 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTCTGGTAAGCGAGGCT |
| Suggested primer 2: | GGCTTCTGTTCTGGAGCAGT |