| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.019528 |
| Chromosome: | chromosome 6 |
| Location: | 124871 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g249900 | FAP75 | (1 of 1) PTHR23359//PTHR23359:SF81 - NUCLEOTIDE KINASE // ADENYLATE KINASE 8; Flagellar Associated Protein 75 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCTGTCCTGGCTTCCCAGGCGGGGGCGAAGGCGCTGCCGCTGCGGCCG |
| Internal bar code: | GCCTCGATTACCGGGGTTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1001 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATTCCCAATAGCTGTGC |
| Suggested primer 2: | CTCGGCTGCACGAAATGAAG |