| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019533 |
| Chromosome: | chromosome 12 |
| Location: | 8292451 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551250 | FLS1 | Flagellar shortening 1; (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGATGCCTATGCTCCGTGCGACGGCCGGCCATGTGGGTTCCTACCC |
| Internal bar code: | ATTTGTTTATCATTTTTTTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4883 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGGGTCTACCGTAACCT |
| Suggested primer 2: | GGGTTTGGGTCAACATTGGC |