| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.019559 |
| Chromosome: | chromosome 1 |
| Location: | 4273375 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800084 | (1 of 7) PF00628//PF00665 - PHD-finger (PHD) // Integrase core domain (rve) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGCGACACCGCACGCCTCCGTGCGCGAGTGGAGCAGTTGGTGCCGTG |
| Internal bar code: | ATGTTGACTATGTCAAGTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1865 |
| LEAP-Seq percent confirming: | 4.16667 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACTGCCCGTACAACTGA |
| Suggested primer 2: | CCTCAAGCTTCAACCCGCTA |