| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019562 |
| Chromosome: | chromosome 4 |
| Location: | 2850799 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224250 | FAP147 | Flagellar Associated Protein 147; (1 of 1) PF14646 - MYCBP-associated protein family (MYCBPAP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATGGGTTGCGTCGTTGGTAGCCGTACACGAAGGATGGGAGGGGCAGG |
| Internal bar code: | CGTGGGACAGGGAACCACTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1177 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCACCTCTTCAACCATGA |
| Suggested primer 2: | ACACACACACACACACACCT |