Insertion junction: CLIP2.019576_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.019576
Chromosome:chromosome 9
Location:6468730
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g415300 GT90-19,GT90F19 GT90 family protein 19; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCAATGAACCATTGCTGCTCCCCCAACAGCGCGGCCTGCACCCGGT
Internal bar code:GCTGGTTTCAGTCGGGTGGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1708
LEAP-Seq percent confirming:96.4286
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGAAACCGTCGTGCTGAT
Suggested primer 2:GATGCGCTTCAGCTCCATTG