Insertion junction: CLIP2.019577_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.019577
Chromosome:chromosome 9
Location:756215
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g404201 PHC30,PHC24,PHC10 Pherophorin-chlamydomonas homolog 30; (1 of 71) PF12499 - Pherophorin (DUF3707) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAATCCCGAACCGCCAGCACCACCATATCCATCATCACCACCGTTTCC
Internal bar code:ATTGAGGAACTGGGGTTTGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:98
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCAGTACTGCTTCACCAT
Suggested primer 2:ACGGGTAACATGGACTGCAG