Insertion junction: CLIP2.019601_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.019601
Chromosome:chromosome 6
Location:2565396
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g269601 (1 of 3) 2.4.1.19 - Cyclomaltodextrin glucanotransferase / Cyclodextrin glycosyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGTCGCGCTGCCTGCTGCGGTGGCCGTCGAGGTCAGCGACTCGTGGGA
Internal bar code:ACGTCGGTTGCCTCGGTGCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2777
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGCGGTTTGACAGTGAC
Suggested primer 2:GTCACTGTCAAACCGCACAC